Comparative Impact of Bacterial and Yeast Probiotic Strains in Functional Foods: Mechanisms, Efficacy and Applications
DOI:
https://doi.org/10.62051/xep28116Keywords:
Bacterial; Yeast Probiotic Strains; Functional Foods.Abstract
This review systematically examines four principal probiotic groups in the context of functional food applications: Lactobacillus, represented by L. rhamnosus GG (LGG), which enhances epithelial barrier integrity via SpaCBA pili-mediated adhesion and secreted proteins Msp1/p75 and Msp2/p40 that activate EGFR-Akt signaling to attenuate cytokine-induced apoptosis; Bifidobacterium, which mediates ecosystem-level colonic regulation through acetate-driven cross-feeding with butyrate-producing taxa and strain-specific immunomodulation including NF-κB suppression and IL-10 induction; Streptococcus thermophilus, whose high β-galactosidase activity directly hydrolyzes lactose during intestinal transit, with clinical evidence confirming viability-dependent reduction in breath-hydrogen excretion and elevation of plasma butyrate; and Saccharomyces boulardii, a eukaryotic probiotic resilient to many antibiotics that mediates rapid, transient anti-diarrheal effects through serine protease-mediated toxin degradation, mucosal IgA stimulation, and MAP kinase-dependent suppression of pro-inflammatory mediators, with clinical trials confirming significant reductions in antibiotic-associated diarrhea incidence across adult and pediatric populations. Translational trends toward postbiotics and personalized nutrition are highlighted, alongside persistent challenges including heterogeneity in strain annotation and dosing, variability in host responses, and the paucity of long-term safety data. Three priority directions are identified: systematic clinical validation of strain-specific effects and dose–response relationships; development of standardized pipelines for personalized probiotic recommendations integrating microbiome diagnostics with rigorous clinical evidence; and cautious exploration of engineered probiotic chassis and metabolite-based products with explicit assessment of technical feasibility, regulatory pathways and safety.
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[1] Arzamasov, A. A., Rodionov, D. A., Hibberd, M. C., Guruge, J. L., Kent, J. E., Kazanov, M. D., Leyn, S. A., Elane, M. L., Sejane, K., Furst, A., Bode, L., Barratt, M. J., Gordon, J. I., & Osterman, A. L. (2025). Integrative genomic reconstruction reveals heterogeneity in carbohydrate utilization across human gut bifidobacteria. Nature Microbiology. https://doi.org/10.1038/s41564-025-02056-x DOI: https://doi.org/10.1101/2024.07.06.602360
[2] Gasbarrini, G., Bonvicini, F., & Gramenzi, A. (2016). Probiotics History. Journal of Clinical Gastroenterology, 50, S116–S119. https://doi.org/10.1097/mcg.0000000000000697 DOI: https://doi.org/10.1097/MCG.0000000000000697
[3] Giraffa, G., Chanishvili, N., & Widyastuti, Y. (2010). Importance of lactobacilli in food and feed biotechnology. Research in Microbiology, 161(6), 480–487. https://doi.org/10.1016/j.resmic.2010.03.001 DOI: https://doi.org/10.1016/j.resmic.2010.03.001
[4] Martens, E. C., Neumann, M., & Desai, M. S. (2018). Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier. Nature Reviews Microbiology, 16(8), 457–470. https://doi.org/10.1038/s41579-018-0036-x DOI: https://doi.org/10.1038/s41579-018-0036-x
[5] Segers, M. E., & Lebeer, S. (2014). Towards a better understanding of Lactobacillus rhamnosus GG - host interactions. Microbial Cell Factories, 13(Suppl 1), S7. https://doi.org/10.1186/1475-2859-13-s1-s7 DOI: https://doi.org/10.1186/1475-2859-13-S1-S7
[6] Karczewski, J., Troost, F. J., Konings, I., Dekker, J., Kleerebezem, M., Brummer, R.-J. M., & Wells, J. M. (2010). Regulation of human epithelial tight junction proteins by Lactobacillus plantarum in vivo and protective effects on the epithelial barrier. American Journal of Physiology. Gastrointestinal DOI: https://doi.org/10.1152/ajpgi.00327.2009
[7] Lebeer, S., Vanderleyden, J., & De Keersmaecker, S. C. J. (2010). Host interactions of probiotic bacterial surface molecules: comparison with commensals and pathogens. Nature Reviews Microbiology, 8(3), 171–184. https://doi.org/10.1038/nrmicro2297 DOI: https://doi.org/10.1038/nrmicro2297
[8] Donato, K. A., Gareau, M. G., Wang, Y. J. J., & Sherman, P. M. (2010). Lactobacillus rhamnosus GG attenuates interferon-{gamma} and tumour necrosis factor-alpha-induced barrier dysfunction and pro-inflammatory signalling. Microbiology (Reading, England), 156(Pt 11), 3288–3297. https://doi.org/10.1099/mic.0.040139-0 DOI: https://doi.org/10.1099/mic.0.040139-0
[9] Xiao, M., Zhang, C., Duan, H., Arjan Narbad, Zhao, J., Chen, W., Zhai, Q., Yu, L., & Tian, F. (2024). Cross-feeding of bifidobacteria promotes intestinal homeostasis: a lifelong perspective on the host health. Npj Biofilms and Microbiomes, 10(1). https://doi.org/10.1038/s41522-024-00524-6 DOI: https://doi.org/10.1038/s41522-024-00524-6
[10] Fukuda, S., Toh, H., Hase, K., Oshima, K., Nakanishi, Y., Yoshimura, K., Tobe, T., Clarke, J. M., Topping, D. L., Suzuki, T., Taylor, T. D., Itoh, K., Kikuchi, J., Morita, H., Hattori, M., & Ohno, H. (2011). Bifidobacteria can protect from enteropathogenic infection through production of acetate. Nature, 469(7331), 543–547. https://doi.org/10.1038/nature09646 DOI: https://doi.org/10.1038/nature09646
[11] Groeger, D., Yao, L., Collins, F., Larsen, I. S., Tan, H.-T. T., Healy, S., Ambrogi, V., Tykwinska, K., Schmidt, M., Golletz, P., Kiely, B., Clarke, G., Dinan, T. G., Murphy, E. F., & O’Mahony, L. (2025). Complementary immunoregulatory effects of Bifidobacterium longum 1714TM associated exopolysaccharide and tryptophan metabolism. Current Research in Microbial Sciences, 9, 100481. https://doi.org/10.1016/j.crmicr.2025.100481 DOI: https://doi.org/10.1016/j.crmicr.2025.100481
[12] Sharma, A., Sharma, G., & Im, S.-H. (2025). Gut microbiota in regulatory T cell generation and function: mechanisms and health implications. Gut Microbes, 17(1). https://doi.org/10.1080/19490976.2025.2516702 DOI: https://doi.org/10.1080/19490976.2025.2516702
[13] Pan, Y., Yu, P., Jiang, Y., Liu, X., Zhao, J., Zhang, H., & Chen, W. (2023). The protective effect of lactose on the bile salt stress response of Streptococcus thermophilus is strain dependent. Food Bioscience, 53, 102560–102560. https://doi.org/10.1016/j.fbio.2023.102560 DOI: https://doi.org/10.1016/j.fbio.2023.102560
[14] Yu, P., Pan, Y., Pei, Z., Guo, M., Yang, B., Lee, Y.-K., Liu, X., Zhao, J., Zhang, H., & Chen, W. (2023). Influence of Lactose Supplementation on Regulation of Streptococcus thermophilus on Gut Microbiota. Nutrients, 15(22), 4767–4767. https://doi.org/10.3390/nu15224767 DOI: https://doi.org/10.3390/nu15224767
[15] Rizkalla, S. W., Luo, J., Kabir, M., Chevalier, A., Pacher, N., & Slama, G. (2000). Chronic consumption of fresh but not heated yogurt improves breath-hydrogen status and short-chain fatty acid profiles: a controlled study in healthy men with or without lactose maldigestion. The American Journal of Clinical Nutrition, 72(6), 1474–1479. https://doi.org/10.1093/ajcn/72.6.1474 DOI: https://doi.org/10.1093/ajcn/72.6.1474
[16] Nam, E. H., Lee, M., Kim, H., Kim, D., Lee, Y., Jung, Y. H., Yang, J., & Shin, M. (2025). The impact of Streptococcus thermophilus IDCC 2201 on gut microbiota and its potential as a prophylactic agent for colorectal cancer. Scientific Reports, 15(1). https://doi.org/10.1038/s41598-025-20976-w DOI: https://doi.org/10.1038/s41598-025-20976-w
[17] Guarner, F., Perdigon, G., Corthier, G., Salminen, S., Koletzko, B., & Morelli, L. (2005). Should yoghurt cultures be considered probiotic? British Journal of Nutrition, 93(6), 783–786. https://doi.org/10.1079/bjn20051428 DOI: https://doi.org/10.1079/BJN20051428
[18] Kelesidis, T., & Pothoulakis, C. (2012). Efficacy and safety of the probiotic Saccharomyces boulardii for the prevention and therapy of gastrointestinal disorders. Therapeutic Advances in Gastroenterology, 5(2), 111–125. https://doi.org/10.1177/1756283X11428502 DOI: https://doi.org/10.1177/1756283X11428502
[19] Castagliuolo, I., Riegler, M. F., Valenick, L., LaMont, J. T., & Pothoulakis, C. (1999). Saccharomyces boulardii Protease Inhibits the Effects of Clostridium difficile Toxins A and B in Human Colonic Mucosa. Infection and Immunity, 67(1), 302–307. https://doi.org/10.1128/iai.67.1.302-307.1999 DOI: https://doi.org/10.1128/IAI.67.1.302-307.1999
[20] Dmitrii Manshin, Kuntsova, M., Alexey Shilenko, & Andreeva, A. (2025). Probiotic yeast Saccharomyces cerevisiae var. boulardii: properties and peculiarities of use in functional foods development. Functional Foods in Health and Disease, 15(5), 296–315. https://doi.org/10.31989/ffhd.v15i5.1482 DOI: https://doi.org/10.31989/ffhd.v15i5.1482
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